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sirna targeting rest  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology sirna targeting rest
    Sirna Targeting Rest, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sirna+targeting+rest/NRSF+siRNA/pm41606712-62-8-11
    Average 94 stars, based on 1 article reviews
    sirna targeting rest - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Proliferation of activated hepatic stellate cells requires REST.
    Article Snippet: Primary human hepatic stellate cells Primary human hepatic stellate cells were purchased from LifeNet Health (USA), thawed, seeded at the density of 5000 cells/cm2, and cultured according to manufacturer’s instructions. .. Cells were transfected with scrambled siRNA (ThermoFisher, #4390843, Waltham, Massachusetts, USA) or siRNA targeting REST (Santa Cruz, #sc-38129, Dallas, Texas, USA) using reverse transfection with RNAiMAX (ThermoFisher, #13778- 150). .. Complexes were prepared in Opti-MEM (ThermoFisher, #31985-047) and placed AR TIC LE IN PR ES S in culture plates.

    Article Title: Proliferation of activated hepatic stellate cells requires REST.
    Article Snippet: .. Cells were transfected with scrambled siRNA (ThermoFisher, #4390843), siRNA targeting REST (Santa Cruz, #sc-38129), siRNA targeting phosphatidylinositol 3- kinase regulatory subunit α (PIK3R1) (ThermoFisher, #AM6708, assay ID 118085) or siRNA targeting regulatory-associated protein of mTOR (RAPTOR) (ThermoFisher, #AM6708, assay ID 140660) using reverse transfection as described above. ..



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    PCCN cells are transfected with a control vector (HA-vector), REST siRNA or REST vector to knockdown or overexpress REST, respectively, and then exposed to PrP106-126 for 24 h. a. - e. TUNEL assay shows increased apoptosis in cells with REST knockdown and decreased apoptosis with REST overexpression. Apoptotic cells are labelled with the in situ cell death kit, POD and counterstained with propidium iodide (red). DNase I = positive control. Scale bar = 50μm. f. - j. Hoechst 33258 staining shows that REST overexpression rescued cells from nuclear fragmentation and condensation in response to PrP106-126. Staurosporine = positive control. Scale bar = 5μm. k. Flow cytometry analysis shows REST overexpression reduces apoptosis of both early and late stages induced by the prion peptide. Staurosporine = positive control. l. Immunoblotting analysis of REST in PCCN. m. , n. Inhibition of cell apoptosis (m) and increased cell survival (n) of PCCN by REST overexpression and treated with PrP106-126 were confirmed by flow cytometry. Data are mean ± S.D. of three independent experiments. **P < 0.01 and && P < 0.01 both versus cells treated without PrP106-126; ## P < 0.01 versus cells transfected with REST-vector.

    Journal: Oncotarget

    Article Title: REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-β-catenin signaling

    doi: 10.18632/oncotarget.7640

    Figure Lengend Snippet: PCCN cells are transfected with a control vector (HA-vector), REST siRNA or REST vector to knockdown or overexpress REST, respectively, and then exposed to PrP106-126 for 24 h. a. - e. TUNEL assay shows increased apoptosis in cells with REST knockdown and decreased apoptosis with REST overexpression. Apoptotic cells are labelled with the in situ cell death kit, POD and counterstained with propidium iodide (red). DNase I = positive control. Scale bar = 50μm. f. - j. Hoechst 33258 staining shows that REST overexpression rescued cells from nuclear fragmentation and condensation in response to PrP106-126. Staurosporine = positive control. Scale bar = 5μm. k. Flow cytometry analysis shows REST overexpression reduces apoptosis of both early and late stages induced by the prion peptide. Staurosporine = positive control. l. Immunoblotting analysis of REST in PCCN. m. , n. Inhibition of cell apoptosis (m) and increased cell survival (n) of PCCN by REST overexpression and treated with PrP106-126 were confirmed by flow cytometry. Data are mean ± S.D. of three independent experiments. **P < 0.01 and && P < 0.01 both versus cells treated without PrP106-126; ## P < 0.01 versus cells transfected with REST-vector.

    Article Snippet: Small interfering siRNA targeting REST (Cat. No. 1027416, Qiagen, Valencia, CA, USA) was used for silencing REST expression.

    Techniques: Transfection, Control, Plasmid Preparation, Knockdown, TUNEL Assay, Over Expression, In Situ, Positive Control, Staining, Flow Cytometry, Western Blot, Inhibition

    PCCN cells are transfected with the control vector (HA-vector) without exposure to PrP106-126 a. - c. , or transfected with the control vector j. - l. , REST siRNA d. - f. or REST vector g. - i. and then exposed to PrP106-126 for 24 h as described in Material and Methods. Transmission electron microscopy shows normal morphology of nucleus (light blue arrows) (a) and mitochondria (white arrows) (b, c) in the negative control, and various sizes of vacuoles or swollwn and disorganized smooth endoplasmic reticulum (green arrows) as well as swollen and vacuolization of mitochondria (red arrows) in siRNA REST (d, e) or HA-vector (k) transfected cells. Black arrows or blue arrows indicate the presence of myelin figures (f) or nuclear fragmentation (l). PCCN transfected with REST and then exposed to PrP106-126 show normal morphology of organelles and barely visible vacuolization with only slightly swollen mitochondria (h, i).

    Journal: Oncotarget

    Article Title: REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-β-catenin signaling

    doi: 10.18632/oncotarget.7640

    Figure Lengend Snippet: PCCN cells are transfected with the control vector (HA-vector) without exposure to PrP106-126 a. - c. , or transfected with the control vector j. - l. , REST siRNA d. - f. or REST vector g. - i. and then exposed to PrP106-126 for 24 h as described in Material and Methods. Transmission electron microscopy shows normal morphology of nucleus (light blue arrows) (a) and mitochondria (white arrows) (b, c) in the negative control, and various sizes of vacuoles or swollwn and disorganized smooth endoplasmic reticulum (green arrows) as well as swollen and vacuolization of mitochondria (red arrows) in siRNA REST (d, e) or HA-vector (k) transfected cells. Black arrows or blue arrows indicate the presence of myelin figures (f) or nuclear fragmentation (l). PCCN transfected with REST and then exposed to PrP106-126 show normal morphology of organelles and barely visible vacuolization with only slightly swollen mitochondria (h, i).

    Article Snippet: Small interfering siRNA targeting REST (Cat. No. 1027416, Qiagen, Valencia, CA, USA) was used for silencing REST expression.

    Techniques: Transfection, Control, Plasmid Preparation, Transmission Assay, Electron Microscopy, Negative Control